a, HeLa cells treated with non-targeting (NT) or αTAT1-specific siRNAs were assessed for endocytosis of Alexa488-transferrin (Tf). Data expressed as average percentage ± s.e.m of surface bound Tf at time 0. b, HeLa cells treated with non-targeting (NT) or αTAT1-specific siRNAs and overexpressing GFP-EB1 and mRFP-LCa were imaged by TIRF-M (for 120 s at 1 frame/s). The percentage of EB1 comets that stopped upon contact with CCPs was quantified and compared with randomized scenarios obtained by shifting the LCa mask of 5 pixels in all four orthogonal directions (N, number of cells analysed; n, number of EB1 comets analysed; * P<0.001, ns: no significant difference).