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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: J Immunol. 2014 Feb 12;192(6):2812–2820. doi: 10.4049/jimmunol.1301794

FIGURE 7.

FIGURE 7

MC degranulation after thermal challenge is preserved in mMCP5−/− mice. (A) Histological demonstration (CAE reactivity) of degranulating MCs in the skin of an mMCP5−/− mouse (arrow indicates extruded granules). Insert shows an intact MC from mMCP5−/− mouse skin (magnification 630×). (B) MC degranulation in mMCP5−/− mice shows similar percentage to that observed in WT mice (mean ± SEM, n=4–5 mice per time point from 2 experiments). (C) EM of a mMCP5−/− MC captured 120 s after scald injury (magnification 12,000×) demonstrating zonal MC degranulation (box). Insert shows higher magnification of degranulation (magnification 30,000×). (D, E) PMN margination (D) and tissue influx (E) in thermally challenged skin of mMCP5−/− and WT mice (mean ± SEM, n=4–7 mice per time point from 3 experiments).