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. 2014 Feb 18;111(12):4638–4643. doi: 10.1073/pnas.1323063111

Fig. 5.

Fig. 5.

(A) BN gel analysis of HT3PSII, HT3∆QPSII, and HT3∆OPSII. Dimeric (marked as “D”) and monomeric (marked as “M”) PSII are indicated. Note the increase in RC47 (CP43-less monomeric PSII) in HT3∆OPSII. (B) Polypeptide profiles of isolated HT3PSII, HT3∆QPSII, and HT3∆OPSII. The positions of the major PSII protein components are indicated on the left. PsbO position is marked with an asterisk. Each lane was loaded with sample containing 5 µg of Chla. (C) Immunodetection of PSII polypeptides in the isolated complexes. Samples containing 0.2 µg of Chla were fractionated by SDS/PAGE, transferred to a PVDF membrane, and probed by using anti-CP47, anti-PsbO, and anti-PsbQ antibodies.