Fig. 4.

MiR-21 promotes cell death on primary myoblasts. (A) TLR7^+/+ and TLR7^−/− primary myoblasts were treated with Dotap formulations of miR-16 and miR-21 for 24 h, and then cell viability was assessed with a cell counter. As negative control, cells were incubated with Dotap alone. (B) Determination of live primary myoblasts after incubation for 24 h with MVs isolated from A549 cells previously transfected with LNA-antinegative control and LNA-anti-miR-21. Experiments in A and B were performed in quadruplicate. Results are presented as average ± SEM. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.