Fig. 1.

Efficient production of transgene-free Arabidopsis plants with desired modifications at the targeted loci. Arabidopsis flowers in the T0 generation are infiltrated with Agrobacteria that carry the transgenes for the CRISPR/Cas system and/or a template for modification. T1 plants carrying the transgenes are selected using an appropriate selection marker. Transgene-free plants with the desired modifications (solid blue colored seedling without a purple circle) can be obtained in the T2 generation. A purple circle indicates seeds, seedlings, or flowers that carry transgenes in the genome. Green, blue, red, and yellow refer to the cells that carry WT copy, desired modification, mutation 1, and mutation 2 at the targeted gene locus, respectively. There can be additional mutations in the population that are not shown in the figure. Solid colors indicate homozygous for the particular alleles. Stripped colors refer to heterozygous for the particular gene.