Fig. 3.
VCP746 is a biased agonist. (A) Effects of selected ligands on A1AR-mediated inhibition of (Left) forskolin-stimulated cAMP accumulation or (Right) ERK1/2 phosphorylation (pERK1/2) in CHO FlpIn cells stably expressing the human A1AR. (B) Bias plots showing the effects of equimolar agonist concentrations at the two pathways. (C) Quantification of bias factors using R-PIA as the reference agonist. Data represent the mean of three experiments ± SEMs performed in duplicate. *P < 0.05; Student's t test.