Fig. 4.

Knockdown of linc-RoR decreases tumor cell viability during hypoxia. (A) HepG2 cells were seeded (1×10⁴/well) into 96-well collagen-coated plates and cultured under conditions of normoxia or hypoxia, and cell viability was assessed after 24 or 48 hours. (B) HepG2 cells or PLC-PRF-5 cells were transfected with two different siRNAs to linc-RoR (1 or 2) or nontargeting control siRNA. After 48 hours, RNA was isolated and qPCR for linc-RoR was performed. (C,D) HepG2 cells were transfected with siRNA 1 or 2 to linc-RoR (C and D, respectively) or nontargeting control. After 24 hours, cells were plated (1×10⁴/well) in 96-well plates and cultured under normoxia or hypoxia conditions. Cell viability was assessed after 24 or 48 hours and is expressed relative to controls.