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. 2013 Aug;108(5):665–667. doi: 10.1590/0074-0276108052013021

Presence of Leishmania RNA virus 1 in clinical samples. Representative nested retro-transcription polymerase chain reaction (PCR) ethidium bromide stained 2.5% agarose gel. Ladders are 50 bp (left) and 100 bp (right). A: first round PCR using pair 1 primers; B: second and nested round using pair 2 primers; -: negative control; +: positive control with Leishmania (V.) guyanensis (MHOM/BR/1989/IM3597) cDNA; -*: double negative control using first round negative control as template; Lanes 1-3: state of Rio de Janeiro (RJ)-recidivant localised cutaneous leishmaniasis; 4, 8, 9: state of Amazonas-localised cutaneous leishmaniasis (LCL); 5-7: RJ-LCL; 10: RJ-scar; 11: RJ-mucosal leishmaniasis.

Presence of Leishmania RNA virus 1 in clinical samples. Representative nested retro-transcription polymerase chain reaction (PCR) ethidium bromide stained 2.5% agarose gel. Ladders are 50 bp (left) and 100 bp (right). A: first round PCR using pair 1 primers; B: second and nested round using pair 2 primers; -: negative control; +: positive control with Leishmania (V.) guyanensis (MHOM/BR/1989/IM3597) cDNA; -*: double negative control using first round negative control as template; Lanes 1-3: state of Rio de Janeiro (RJ)-recidivant localised cutaneous leishmaniasis; 4, 8, 9: state of Amazonas-localised cutaneous leishmaniasis (LCL); 5-7: RJ-LCL; 10: RJ-scar; 11: RJ-mucosal leishmaniasis.