Products from polymerase chain reaction (PCR) multiplex assays submitted to hybridisation analysis for the diagnosis of naturally Leishmania (Viannia) braziliensis infection in Psychodopygus complexus and Psychodopygus ayrozai collected in the municipality of Guaraí, state of Tocantins, Brazil. A: ethidium bromide-stained 2% agarose gel revealing the 220 bp product form the cacophony gene amplification and the 120 bp fragment corresponding to the conserved region of kinetoplast minicircles from Leishmania spp [M: molecular weight marker (100 bp DNA ladder); Lane 1: amplification reaction without added DNA (PCR negative control); 2-4: negative controls for the DNA extraction step (male insect pools): 5-8: female sandfly pools (5-7: Ps. complexus positive pools; 8: Ps. ayrozai positive pool); 9: PCR positive control (DNA extracted from a mixture of male insect pool containing L. (V.) braziliensis promastigotes)]; B: dot hybridisation using a biotinylated probe specific for parasites from the Viannia subgenus.