TABLE V. Phlebotomine molecular diagnostic evaluation for infection with Leishmania (Viannia) braziliensis and positive polymerase chain reaction hybridisation results with female pools of Psychodopygus complexus and Psychodopygus ayrozai by traps in the municipality of Guaraí, state of Tocantins, Brazil.

Traps	Ps. complexus (p/n)	Ps. ayrozai (p/n)	Females (p/n)
CDC light - HP model	1/52	0/0	2/5
Shannon	2/151	1/51	2/20
Total	3/203	1/51	4/25

the numbers in superscript represent the groups of females positive related to each species of phlebotomine. n: total number of females evaluated; p: number of positive females.

The municipality of Guaraí, state of Tocantins, Brazil.

Monitoring stations (MS) in rural settlement area, Agricultural Project Pedra Branca, municipality of Guaraí, state of Tocantins, Brazil. A, B: MS 1; C, D: MS 2.

Monitoring stations (MS) in periurban area, municipality of Guaraí, state of Tocantins, Brazil. A, B: MS 3; C, D: MS 4.

Products from polymerase chain reaction (PCR) multiplex assays submitted to hybridisation analysis for the diagnosis of naturally Leishmania (Viannia) braziliensis infection in Psychodopygus complexus and Psychodopygus ayrozai collected in the municipality of Guaraí, state of Tocantins, Brazil. A: ethidium bromide-stained 2% agarose gel revealing the 220 bp product form the cacophony gene amplification and the 120 bp fragment corresponding to the conserved region of kinetoplast minicircles from Leishmania spp [M: molecular weight marker (100 bp DNA ladder); Lane 1: amplification reaction without added DNA (PCR negative control); 2-4: negative controls for the DNA extraction step (male insect pools): 5-8: female sandfly pools (5-7: Ps. complexus positive pools; 8 : Ps. ayrozai positive pool); 9: PCR positive control (DNA extracted from a mixture of male insect pool containing L. (V.) braziliensis promastigotes)]; B: dot hybridisation using a biotinylated probe specific for parasites from the Viannia subgenus.