TABLE I. Total lipids synthesised by posterior midgut after contact with ³ H-palmitic acid at the luminal side.

	cpm/midgut	(%)
TG	450 ± 98	3
DG	2,200 ± 456	16
PC	6,870 ± 295	49
PE	4,390 ± 372	32
Total	13,910 ± 2,212	100

the posterior midguts of adult females were dissected on the 10th day after blood feeding and ³ H-palmitic acid was added at luminal side and incubated in culture medium in the presence of lipophorin. Thirty minutes later, the posterior midgut was washed and subjected to a lipid extraction and the lipids were analysed by thin-layer chromatography. The spots were removed and eluted and the radioactivity associated with each lipid was estimated by scintillation counting. cpm: counts per minute; DG: diacylglycerols; PC: phosphatidylcholine; PE: phosphatidylethanolamine; TG: triacylglycerol.