Fig. 1. Various glutamate-rich protein (GLURP) fragments amplified by nested polymerase chain reaction from Plasmodium falciparum isolates from Porto Velho, state of Rondônia (RO), Brazil. C-: without DNA; C+: with DNA extracted from a culture of P. falciparum (FCR3); M: 200-bp marker; R0a: 1,400 bp; R2a: 750 bp; R2b: 800 bp; R2c: 950 bp; R2d: 1,100 bp.