Fig. 1: Lanes 1-14 in A and B contain the same Trypanosoma cruzi I strains or clones. A: differentiation between Trypanosoma cruzi I and T. cruzi II using TCC/TC1/TC2 primers (Souto et al. 1996). 1-14: T. cruzi I (350 bp); 15: T. cruzi II (300 bp); M: 100 bp ladder; 1: MHOM/C0/04/MG strain; 2: CG clone 1; 3: ORT15 Rcol strain; 4: Rcol clone 03; 5: Rpal clone 139-2; 6: Rpal clone 89-2; 7: Rpro (sylvatic) clone 8; 8: Rpro (sylvatic) clone 10; 9: D1 ( Didelphis ) strain; 10: D2 ( Didelphis ) strain; 11: X1081 Rpro (domestic) strain; 12: X1082 Rpro (domestic) strain; 13: CG clone 13; 14: EH strain; 15: T. cruzi II (300 bp); B: genotyping T. cruzi I strains and clones using a single pair of primers (1Am/1B) designed in the spliced-leader intergenic region. Domestic strains were identified by amplifying a 231 bp fragment and sylvatic strains were identified by amplifying a 450-550 bp fragment. M: 100 bp ladder; 1: MHOM/C0/04/MG strain; 2: CG clone 1; 3: ORT15 Rcol strain; 4: Rcol clone 03; 5: Rpal clone 139-2; 6: Rpal clone 89-2; 7: Rpro (sylvatic) clone 8; 8: Rpro (sylvatic) clone 10; 9: D1 ( Didelphis ) strain; 10: D2 ( Didelphis ) strain; 11: X1081 Rpro (domestic) strain; 12: X1082 Rpro (domestic) strain; 13: CG clone 13; 14: EH strain; M: 100 bp ladder.