Fig. 1: proportion of ECwt-infected cells in small intestinal villi. A: ICR mice were inoculated with ECwt (8 x 10 4 focus forming units/mL) and their intestinal villi isolated from the entire small intestine after three days post-inoculation (d.p.i.) For immunochemistry ana-lysis, villi were fixed with ice-cold methanol-acetic acid and reacted with rabbit polyclonal antibodies (Abs) against rotavirus structural proteins (SP). The reaction of villus-associated cells from different mice (1-6) was detected using horseradish peroxidase-conjugate goat anti-rabbit Ab and aminoethylcarbazole substrate. Graph shows significant ECwt infection as compared with uninfected control mice (p < 0.0001); B: small intestinal villi isolated at one, two, three, four and five d.p.i. from ECwt-infected mice (n = 10 mice distributed in 5 days) were fixed with methanol-acetic acid and treated with polyclonal Abs against rotavirus SP before detecting the reaction as in indicated in A. Non-infected mice were used as a control. Photographs from 10 representative fields were analysed for each experimental villus intestinal epithelial cells sample. Data are expressed as percentage ± standard deviation (p < 0.002) of villus-associated cells being positive to ECwt SP relative to the total cells analysed.