Phenotypic and functional analyses of red blood cells (RBCs) derived from CD34 + human haematopoietic stem cells (hHSCs) of peripheral blood (PB) and bone marrow (BM). A: differentiation of hHSCs observed on bright field after cytospin and staining with Giemsa and Brilliant cresyl blue at the indicated days. Magnitude 1,000X; B: analysis of haemoglobin by Maldi TOF Mass Spectrometry [human control RBCs (red), reticulocytes from BM CD34 + cells (blue), reticulocytes from human umbilical cord CD34 + cells (green)]; C: flow cytometry analysis of day 14 reticulocytes derived from PB CD34 + hHSCs and differentiated either with 3T3 or MS5 feeder stromal cells. Cells were double labelled with antibodies against glycophorin A (GlyA-APC) and Duffy blood receptor (Duffy-PE); D: Giemsa stained smears of RBCs derived from PB CD34 + hHSCs infected with Plasmodium falciparum (1) and Plasmodium vivax (2) after 24 h post infection.