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. Author manuscript; available in PMC: 2014 Mar 31.
Published in final edited form as: Acta Biomater. 2010 Jan 22;6(7):2621–2630. doi: 10.1016/j.actbio.2010.01.023

Figure 3.

Figure 3

Dendritic cells purified by magnetic sorting were less responsive to LPS stimulation in comparison to unpurified counterparts. On day 5 of DC culture, DCs were magnetically isolated by removing CD19+ B cells and then positively selecting CD1c+ DCs, treated with LPS for mDCs or left untreated for iDCs. The geometric mean fluorescence intensity (gMFI) of these purified DCs was analyzed by flow cytometry for surface marker expression after 24 hrs and compared to the unpurified counterparts with mean ± range, n=2 donors.