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. Author manuscript; available in PMC: 2014 Mar 31.
Published in final edited form as: Acta Biomater. 2010 Jan 22;6(7):2621–2630. doi: 10.1016/j.actbio.2010.01.023

Table 1.

The comparison between the conventional flow cytometric method and the filter plate-based HTP method.

Flow Cytometry Filter Plate
Plate format 6-well plate 96-well plate
Fixation step No generally Yes
Equipment Flow cytometer Microplate reader
Experimental time 3 hours (1 hr incubation) 2.5 – 3 hours (1.5 hr incubation) for 3 plates
# of materials 5 Up to 279 (or 135 if duplicate) in 3 plates
Size of biomaterial in well 9.5 cm2 0.32 cm2
Data acquisition time 30 – 60 min 30 – 60 second per plate, 2 fluorophores
Data Expression of 6 surface markers with histograms and dot plots A ratio from the average expression of a limited set of surface markers
Marker expression level Very low to high (for markers with any expression level) Relatively high and movable (appropriate for markers with high expression level and huge fold change upon response)