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. Author manuscript; available in PMC: 2015 Feb 14.
Published in final edited form as: Brain Res. 2013 Dec 16;1547:1–15. doi: 10.1016/j.brainres.2013.12.012

Figure 2.

Figure 2

Kv2.1-IR cluster areas decrease in lumbar α-motoneurons following increased in vitro activity. Bath application of either artificial cerebral spinal fluid (ACSF) (panel A) or 10 μM glutamate (panel B) was applied to lumbar spinal cord slices for 10 minutes. Arrowheads point to the large Kv2.1-IR macroclusters. (A) Micrograph representation of single optical confocal section of tissue slice incubated in ACSF. Scale bar is 10 μm. (B) Micrograph representation of single optical confocal section of tissue slice incubated in artificial cerebral spinal fluid with 10 μM glutamate. Scale bar is 10 μm. (C) Quantitative analysis showing significant decrease of Kv2.1-IR macrocluster areas following glutamate treatment. Number of clusters sampled is displayed as “n” from 20 total cells of each treatment group. Data is presented as mean ± SEM.