Time course of Kv2.1-IR declustering of gastrocnemius motoneuron somas following tibial nerve crush at key time points (20 minutes, 3 days, 8 days, 2 weeks, 4 weeks, and 6 months). Kv2.1-IR cluster sizes are still significantly smaller 6 months following injury with reinnervation. All comparisons are made to sham surgery control animals analyzed at 3 day, 8 day and 4 weeks following surgery. No significant differences were found between or within time points for the ipsilateral and contralateral sides in sham surgery control animals. Data is presented as mean ± SEM. Significance (p<0.05) was determined with pairwise ANOVA. (A) Significant decrease (indicated by single asterisk) in Kv2.1-IR areas on injured motoneurons appear as early as 20 minutes following injury and are significantly reduced at all time points following injury. There are also small, yet significant (indicated by double asterisk), decreases on the injury spared contralateral side of the rat spinal cord at 20min, 3 days, 2 weeks and 4 weeks post injury. (B) The number of Kv2.1-IR macroclusters on motoneurons somas decrease significantly (indicated with asterisk) 3 days following tibial nerve crush and are significantly reduced at all following time points.