Table 3.

μCT and histomorphometry of distal femoral cancellous bone. One, 3 and 6 month old Osx-Cre^+/−;Notch1^Δ/Δ;Notch2^Δ/Δ male mice (Notch1/2 Null) or littermate Notch1^loxP/loxP;Notch2^loxP/loxP controls of the same sex (Control).

Male Mice	1 Month		3 Month		6 Month
μCT	Control	Notch1/2 Null	Control	Notch1/2 Null	Control	Notch1/2 Null
Bone Volume/Total Volume (%)	4.6 ± 0.7	4.4 ± 0.5	4.6 ± 0.8	6.7 ± 0.3^*	5.5 ± 0.8	7.0 ± 0.5
Trabecular Separation (μm)	236 ± 12	239 ± 9	262 ± 6	190 ± 2^*	266 ± 9	235 ± 13
Trabecular Number (mm⁻¹)	4.31 ± 0.22	4.26 ± 0.17	3.85 ± 0.87	5.25 ± 0.54^*	3.75 ± 0.11	4.32 ± 0.21^*
Trabecular Thickness (μm)	23.9 ± 0.8	25.1 ± 1.4	32.4 ± 1.5	28.4 ± 1.0^*	33.3 ± 1.4	30.3 ± 2.9
Connectivity Density (mm⁻³)	137 ± 53	147 ± 26	86 ± 26	209 ± 18^*	90 ± 15	231 ± 30^*
Structure Model Index (SMI)	3.06 ± 0.10	3.08 ± 0.07	2.86 ± 0.13	2.64 ± 0.06	2.52 ± 0.15	2.17 ± 0.10⁺
Histomorphometry	Control	Notch1/2 Null	Control	Notch1/2 Null	Control	Notch1/2 Null
Osteoblast Surface/Bone Surface (%)	19.7 ± 2.1	30.9 ± 1.5^*	19.3 ± 1.9	23.7 ± 1.7	19.1 ± 1.9	17.8 ± 2.3
Osteoblasts/Bone Perimeter (mm⁻¹)	16.7 ± 1.7	24.4 ± 0.9^*	16.6 ± 1.7	19.7 ± 1.3	18.1 ± 1.7	16.2 ± 1.8
Osteoid Surface/Bone Surface (%)	3.0 ± 0.5	5.1 ± 0.6^*	3.8 ± 0.7	3.3 ± 0.3	2.5 ± 0.6	2.6 ± 0.7
Osteoclast Surface/Bone Surface (%)	16.9 ± 1.0	13.9 ± 0.9^*	13.5 ± 0.8	15.8 ± 1.0	11.0 ± 1.1	11.0 ± 0.5
Osteoclasts/Bone Perimeter (mm⁻¹)	8.9 ± 0.5	7.3 ± 0.5^*	5.8 ± 0.4	6.8 ± 0.5	5.6 ± 0.8	5.3 ± 0.3
Eroded Surface/Bone Surface (%)	21.5 ± 1.2	18.2 ± 0.8^*	13.6 ± 0.4	15.2 ± 0.9	12.0 ± 2.0	11.5 ± 0.8
Mineral Apposition Rate (μm day⁻¹)	2.68 ± 0.10	2.24 ± 0.06^*	1.85 ± 0.16	1.58 ± 0.08	1.32 ± 0.16	1.52 ± 0.12
Mineralizing Surface/Bone Surface (%)	8.6 ± 1.9	10.3 ± 1.2	6.8 ± 1.3	6.2 ± 0.9	2.9 ± 0.7	4.9 ± 0.8
Bone Formation Rate (μm³ μm⁻²day⁻¹)	0.23 ± 0.05	0.23 ± 0.03	0.13 ± 0.03	0.10 ± 0.02	0.04 ± 0.01	0.08 ± 0.01

Values are means ± SEM; n = 4 - 10.

Significantly different between Notch1/2 Null and Control, p < 0.05.