Figure 2.

Akt activation induced by thrombin is partly dependent of extracellular and intracellular Ca²⁺. Representative Western blots showing the expression level of phosphorylated and total protein of Akt (A) or ERK1/2 (B). All conditions were stimulated by thrombin (100 nmol/L) for 15 min in Ca²⁺‐containing media, Ca²⁺‐free media, and in Ca²⁺‐free media in the presence of 10 μmol/L CPA. Blots are representative of 3 independent sets of experiments.