Figure 3.

Effects of CGP37157 on [Ca²⁺]_c and secretion transients elicited by repeated cell challenging with ACh. Experimental conditions and stimulation with ACh were as in Figure 1, except for the fact here CGP37157 (3 μmol/L) was cell perifused since 1 min before P4 to 1 min after P8 (top horizontal bars). (A) Example record of the [Ca²⁺]_c transients elicited by ACh in a fura‐2‐loaded BCC; inset shows the transients generated by the ACh pulses P4 and P5 at an expanded time scale. (B) Pooled averaged data on the normalized peak [Ca²⁺]_c amplitude, calculated from experiments as that shown in (A). (C) Example catecholamine release responses (in nA, ordinate) obtained from a BCC batch; inset shows the transients elicited by ACh pulses P4 and P5 at an expanded time scale. (D) averaged data from the secretion responses obtained using the protocols of panel (C). Data in (B and D) are means ± SEM of the number of single cells ([Ca²⁺]_c) and cell batches (secretion) from three different cultures, shown in parentheses. *P <0.05, **P <0.01 with respect to P4.