Table 1.
Kinetic parameters of the [Ca2+]c and secretion transient responses elicited by ACh pulses given to perifused BCCs at 37°C, following the protocols shown in Figures 1–3.
| Treatment | Height (peak) * | Width * | Area * | Clearance (τ) * | ||||
|---|---|---|---|---|---|---|---|---|
| [Ca2+]c | Secretion | [Ca2+]c | Secretion | [Ca2+]c | Secretion | [Ca2+]c | Secretion | |
| Control (P4) | 95.6 ± 15.3 | 183 ± 12.5 | 97.8 ± 5.8 | 13.7 ± 0.8 | 91.6 ± 14.3 | 3.39 ± 0.16 | 19.2 ± 1.8 | 18.2 ± 1.2 |
| Control (P5) | 86.3 ± 11.9 | 188 ± 13.2 | 107 ± 6.4 | 13.3 ± 1.3 | 92.8 ± 16.1 | 3.31 ± 0.21 | 20.1 ± 1.9 | 18.3 ± 0.8 |
| Change in P5 with respect to P4 (%) | −9.7 | +2.7 | +9.4 | −2.9 | +1.3 | −2.4 | +4.7 | +0.5 |
| Control (P4) | 88.2 ± 9.4 | 175 ± 38 | 89 ± 9.7 | 12.5 ± 0.9 | 85.9 ± 10.8 | 2.77 ± 0.31 | 12.6 ± 4.4 | 15.7 ± 1.3 |
| Ru360 (P5) | 76.2 ± 8.8 | 465 ± 37* | 136 ± 7.8* | 11.6 ± 0.6 | 54.7 ± 19.7 | 7.1 ± 0.51 | 57.1 ± 10.3* | 12.5 ± 0.8 |
| Change in P5 with respect to P4 (%) | −14 | +166 | +53 | −7.2 | −36 | +256 | +353 | −20 |
| Control (P4) | 91.3 ± 5.7 | 201 ± 11.5 | 106 ± 5.6 | 13 ± 0.6 | 97 ± 6 | 3.65 ± 0.12 | 19.7 ± 2.3 | 11.2 ± 0.5 |
| CGP37157 (P5) | 69.6 ± 5.2* | 163 ± 9.7* | 138 ± 4.7* | 13.4 ± 0.7 | 86 ± 7.1 | 2.99 ± 0.09 | 38.6 ± 3.8* | 11.6 ± 0.5 |
| Change in P5 with respect to P4 (%) | −24 | −19 | +30 | +3 | −11 | −18 | +96 | +4 |
| Control (P4) | 93.7 ± 1.2 | 193 ± 30.2 | 93.3 ± 2.3 | 13.1 ± 1.3 | 92.4 ± 3 | 3.3 ± 0.8 | 18.3 ± 1.6 | 15.7 ± 2.4 |
| Ru360 + CGP37157 (P5) | 71 ± 3.3 | 347 ± 47.8* | 102.2 ± 4.7 | 14.7 ± 0.6 | 77.3 ± 7.6 | 6.3 ± 1.2 | 21.9 ± 3.2 | 15 ± 1.9 |
| Change in P5 with respect to P4 (%) | −24 | +80 | +10 | +12 | −16 | +91 | +20 | −5 |
| Control (P4) | 88.7 ± 1.4 | 188 ± 15.3 | 98.9 ± 5.2 | 13.1 ± 0.5 | 87 ± 3.7 | 3.2 ± 0.3 | 19.3 ± 2.4 | 16.5 ± 1.3 |
| FCCP (P5) | 78.3 ± 8.8 | 424 ± 25.3* | 92.1 ± 6.8 | 13.6 ± 0.6 | 74.7 ± 12.3 | 7.2 ± 0.7* | 9.16 ± 0.9* | 16.1 ± 1.2 |
| Change in P5 with respect to P4 (%) | −12 | +126 | −7 | +4 | −14 | +125 | −53 | −2 |
| Control (P4) | 87 ± 9.4 | 181 ± 8.9 | 96.4 ± 3.1 | 13.2 ± 0.5 | 88.4 ± 9.3 | 2.96 ± 0.07 | 17.5 ± 1.2 | 14.6 ± 0.5 |
| Oligomycin plus rotenone (P5) | 48.9 ± 7.3 | 463 ± 20.6* | 95.5 ± 6.6 | 13.6 ± 0.4 | 88.4 ± 14.3 | 8.34 ± 0.30* | 18.6 ± 2.2 | 13.4 ± 0.4 |
| Change in P5 with respect to P4 (%) | −44 | +156 | −1 | −1 | 0 | +182 | +6 | −8 |
The parameters were calculated as indicated in the insets of Figure 1A and C, and correspond to pulses P4 (control, before giving the compound) and P5 (applied to cells that were perifused with the compounds for 2 min before and during the ACh pulse); drug treatment and % of variation were emphasized in bold.
Height, response amplitude to peak: in the case of [Ca2+]c it was normalized to 100% (Fura‐2 ratios) and in the case of secretion is given as nA current generated by the oxidation of the released catecholamine.
Width of the response transient signal is given in seconds.
Area represents the integrated [Ca2+]c or secretion transients (bulk CRRs) expressed as time‐fold the fura‐2‐fluorescence ratios or as μC (nA × s), respectively.
τ for the clearance (τcl) is the time constant for the decay of the [Ca2+]c and secretion responses given in s−1. Data are means ± SEM (calculated from the experiments of Figs. 1–6). Statistical analyses were carried out with ANOVA one‐way test, and Dunnett′s post hoc test; *P < 0.05 with respect to P1.