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. 2013 Dec 15;1(7):e00181. doi: 10.1002/phy2.181

Figure 3.

Figure 3.

Expression and localization of collagen type IV. (A) Expression of collagen α3(IV) mRNA is upregulated in Smad3 KO TGF‐β1 mice compared with Smad3 KO non‐TGF‐β1 mice (*P = 0.007). A reduction in collagen α3(IV) mRNA is seen in the Smad3 KO non‐TGF‐β1 mice versus Smad3 WT non‐TGF‐β1 mice (†P < 0.05) (n = 6–7/group). (B) Immunofluorescence staining of collagen α4(IV) in glomerulus in Smad3 WT non‐TGF‐β1 (I), Smad3 WT TGF‐β1 (II), Smad3 KO non‐TGF‐β1 (III) and Smad3 KO TGF‐β1 (IV) mice (magnification: 100×) (n = 3/group). (C) Collagen α1/α2(IV) staining (brown) in glomerulus from representative mice; (I) Smad3 WT non‐TGF‐β1, (II) Smad3 WT TGF‐β1, (III) Smad3 KO non‐TGF‐β1, and (IV) Smad3 KO TGF‐β1 mice.