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. 2013 Dec 15;1(7):e00181. doi: 10.1002/phy2.181

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© 2013 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure 10. — Knockdown of Smad3 with siRNA (siSmad3‐2) in murine mesangial and glomerular endothelial cells. In the mesangial cells, real‐time PCR analysis shows that knockdown of Smad3 attenuates TGF‐β1‐induced expression of collagen α1(IV) (B) and MMP‐2 (C) mRNA versus the control (siEGFP) (*P < 0.05), whereas TIMP‐1 (D) is partly blocked (^#P = 0.07) (n = 11–12 wells/treatment/group). In the glomerular endothelial cells, knockdown of Smad3 blocks TGF‐β1‐induced collagen α3(IV) (F), MMP‐2 (G), and MMP‐9 (H) mRNA expression (*P ≤ 0.05) (n = 7 wells/treatment/group). MMP‐2, matrix metalloproteinase‐2; MMP‐9, matrix metalloproteinase‐9; TIMP‐1, tissue inhibitors of metalloproteinase‐1.