Figure 7.

(A) The G₁ block caused by DFO causes apoptosis in neuroblastoma. Confluent serum starved neuroblastoma cells (SKNSH) were subcultured in RPMI with or without DFO. The cells were harvested 2, 3, and 4 days later. For harvesting, the cells were washed with 0.5 mL of cold PBS once, scraped with a rubber policeman in 0.5 mL cold PBS. Twenty microliters of this was diluted to 100 μL using PBS and a subsample of this was counted using the hemacytometer. Another 50 μL was used to conduct cell FACS. Apoptotic cells are observed with DFO 3 days after treatment as a ‘hump’ to the left of the G1 cells (bottom row right). (B) Cells treated with DFO after aphidicolin treatment exhibit an S phase block and exhibit apoptosis a day later. Methods the same as shown in (A).