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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Metabolism. 2013 Jan 26;62(6):873–887. doi: 10.1016/j.metabol.2013.01.001

Fig. 6.

Fig. 6

Proteomic analysis of the upstream molecular signaling pathways for the hepatic expression of p27 in the homozygous leptin-deficient ob/ob mice and homozygous long-lived Ames dwarf mice compared with heterozygous lean and normal control mice, respectively. (A and D) Differential proteomic profiling analysis – by direct matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) – and hematoxylin and eosin (H & E) staining of the cryosectioned liver samples from (A) homozygous leptin-deficient ob/ob mice (n=5 each for experimentals and controls) and (D) homozygous long-lived Ames dwarf mice (n=5 each for experimentals and controls). (B, C, E, and F) Differential proteomic identification analysis – by nano liquid chromatography–tandem mass spectrometry (nano LC-MS/MS) – of the homogenized liver samples from (B and C) homozygous leptin-deficient ob/ob mice (n=5 each for experimentals and controls) and (E and F) homozygous long-lived Ames dwarf mice (n=5 each for experimentals and controls).