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. 2014 Mar 25;2:e323. doi: 10.7717/peerj.323

Figure 2. Identifying the region responsible for foci formation in ataxin-1.

Figure 2

Various deletion constructs of ataxin-1 were expressed as YFP fusion proteins in COS cells. (A) Full-length ataxin-1 30Q-YFP. Overlay with DAPI is shown in B. Expression analysis shows a diffused expression pattern in cells expressing ataxin-1 N terminal regions without (C) or with the polyQ tract (D). In contrast, cells expressing the C-terminal regions either without (E) or with (F) the polyQ tract readily formed foci.