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. 2014 Mar 25;2:e323. doi: 10.7717/peerj.323

Figure 3. AXH domain is not involved in foci formation.

Figure 3

A YFP construct starting at the ataxin-1 AXH domain and ending at the last ataxin-1 amino acid (AXH2End) was expressed as a YFP fusion in COS cells (A and B). These cells showed a diffused YFP fluorescence which was nuclear, as evidenced by DAPI overlay (B). YFP tagged AXH domain also failed to form foci (C and D). Upon N-terminal extension of the AXH2End construct to include further residues starting from amino acids TLND, nuclear foci formation was observed (Figs. 4E and 4F). YFP fluorescence in left panels is overlaid with DAPI in right panels.