Figure 2.

GGCX stability at 25°C monitored by catalytic activity. Activity was measured by the incorporation of radiolabeled ¹⁴C as GGCX carboxylates a small pentapeptide substrate FLEEL in the presence of propeptide, KH₂, oxygen, and NaH¹⁴CO₃. In all sample systems, the 100% active control is recorded at time zero. Stability is compared between microsomal GGCX (red) and GGCX-embedded nanodiscs (blue) with (◊) and without (⧫) pCon. The fractionated proteins from the 24-h time point at 25°C are represented by SDS-PAGE for each sample.