Table 1.
UV Melting and Osmotic Stress of Duplexes Formed by RNA1 and Unmodified Control.
| Sequencea | tm °C | - ΔH kcal/molb |
- ΔH kcal/molc |
- ΔH kcal/mold |
ΔS eud | - ΔG310
kcal/mold |
Ethylene glycol ΔnWe |
Glycerol ΔnWe |
Acetamide ΔnWe |
|---|---|---|---|---|---|---|---|---|---|
| 5′-GCGUaUaUaUCGC 3′-CGCApApApAGCG |
48.6 ± 0.2 | 74.0 ± 3.0 | 86.0 ± 7 | 76.1 ± 2.0 | 209 ± 6 | 11.5 ± 0.3 | 46 ± 3 | 59 ± 5 | 60 ± 5 |
| 5′-GCGUpUpUpUCGC 3′-CGCApApApAGCG |
59.1 ± 0.3 | 87.2 ± 2.7 | 116 ± 19 | 96.6 ± 4.4 | 263 ± 13 | 15.1 ± 0.3 | 52 ± 4 | 63 ± 7 | 66 ± 4 |
a
RNA strands, 2 μM each in 10 mM sodium cacodylate (pH 7.4), 0.1 mM EDTA, and 300 mM NaCl. Results ± standard deviations.
b
From dα/d(1/Tm) vs. Tm.
c
From concentration dependency of Tm.
d
From van’t Hoff analysis.
e
ΔnW = number of waters released upon melting. Results ± error estimates.