Table 1. Comparison of the Activity of MoFe Proteins upon Addition of Isolated FeMoco.
protein | C2H2 reducing activity (nmol mg–1 min–1) | percentage activitya (%) |
---|---|---|
ΔnifH NifDK (as isolated) | 0 | 0 |
ΔnifH NifDK (OAR) | 213 ± 38 | 20 |
ΔnifB NifDK | 1083 ± 138 | 100 |
Percentage activity was calculated by setting the activity of reconstituted ΔnifB NifDK to 100% and comparing the activity of reconstituted ΔnifH NifDK with that of reconstituted ΔnifB NifDK.