Figure 5.

Liposomes encapsulating mitoxantrone uptake in various cell lines followed by confocal laser scanning fluorescence microscopy. Cell lines were treated with either LHRH-MTX-SL or MTX-SL for 4 hours at 37°C. (A) LHRH-MTX-SL in LHRH receptor high-expressing MCF-7 cells; (B) MTX-SL in LHRH receptor high-expressing MCF-7 cells; (C) MCF-7 cells treated with drug-free medium used as a control; (D) LHRH-MTX-SL in LHRH receptor low-expressing SK-OV-3 cells; (E) MTX-SL in LHRH receptor low-expressing SK-OV-3 cells; (F) SKOV-3 cells treated with drug-free medium used as a control.

Note: Copyright © 2010. Reproduced with permission of Dove Medical Press. He Y, Zhang L, Song C. Luteinizing hormone-releasing hormone receptor-mediated delivery of mitoxantrone using LHRH analogs modified with PEGylated liposomes. Int J Nanomedicine. 2010;5:697–705.¹⁰⁸

Abbreviations: LHRH, luteinizing hormone releasing hormone; MTX, methotrexate; SL, loaded liposomes.