Fig. 3.

Schematic of the SPINDLE microscope. The sample in the rose chamber is mounted on a piezo nanopositioner and imaged with a Nikon 1.45, 100X objective and 200 mm Nikon tube lens. The DH-PSF-S is implemented by a 4F relay setup formed by two 100 mm focal length lenses. The DH phase mask is placed at a plane conjugate to the pupil plane of the objective. The final image is formed on the EMCCD. The illumination lasers (641 nm Coherent Cube used for turn off and 488 nm Argon -ion for activation) are used in synchronization with the camera in the scheme shown on the top right.