Fig. 6.

Precision analysis of the SPINDLE data. (a) Precisions per localization in x, y and z directions for different number of photons per image. Theoretical √CRB values calculated for the same system parameters with 30 background photons/pixel and the probability density function for the EMCCD are shown for comparison with the experiment. The theoretical values correspond to √Avg(CRB) for 1.2 µm range. (b) and (c) Histograms of x, y and z estimated positions for molecules detected with 1100 photons and 5000 photons per image, respectively. The measured precision values for (b) and (c) are σ(x,y,z) = (22,29,52) nm and σ(x,y,z) = (7,11,22) nm.