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(a)-(b) Comparison of 3D localization methods via the Average Cramer Rao Bound (CRB3D) over 800 nm and 2 µm ranges with changing background. The calculation parameters for shot-noise limited CRB are 1.45NA, λ = 660 nm, pixel size = 16 µm, 3000 detected photons at focus. (c) shows the CRB for each dimension (x,y and z) and the 3D CRB varying with z for background of 6 photons/pixel.
