Table 1.

Four kinetic equations used in Figure 2. The first order kinetics was chosen since it showed the most reliable pretreatment time-curves based on the values of adjusted R² and probability obtained.

Kinetics	Equation	Adjusted R2	Probability (>F)	t0.5 (min)
Empirical model
Baicalin	$C=187.24-\frac{205.09\times t}{11.16+t}$	0.9586	6.14E-4	11.16
Wogonoside	$C=44.04-\frac{49.77\times t}{33.13+t}$	0.9012	1.30E-3	33.13
Baicalein	$C=79.13+\frac{242.86\times t}{12.08+t}$	0.9380	3.45E-5	12.08
Wogonin	$C=14.36+\frac{57.04\times t}{35.67+t}$	0.8884	2.46E-4	35.67
Zero order
Baicalin	$C=86.86-0.50t$	0.23976	1.00E-1	86.86
Wogonoside	$C=29.39-0.14t$	0.48099	1.50E-2	104.96
Baicalein	$C=119.84+0.60t$	0.25019	1.50E-3
Wogonin	$C=30.43+0.16t$	0.48768	1.60E-3
First order
Baicalin	$C=185.96\times e^{-0.061\times t}$	0.9955	4.61E-7	11.32
Wogonoside	$C=43.7\times e^{-0.025\times t}$	0.9497	5.57E-5	27.69
Baicalein	$C=297.81-218.70\times e^{-0.060\times t}$	0.9796	3.74E-6
Wogonin	$C=61.63-47.49\times e^{-0.027\times t}$	0.9368	7.99E-5
Second order
Baicalin	$C=\frac{1}{6.63\times {10}^{-4}t+1/187.74}$	0.9448	2.36E-4	8.03
Wogonoside	$C=\frac{1}{9.37\times {10}^{-4}t+1/44.47}$	0.9005	2.99E-4	24.01
Baicalein	$C=321.99-\frac{1}{3.41\times {10}^{-4}t+1/242.87}$	0.9380	3.45E-5
Wogonin	$C=71.38-\frac{1}{4.91\times {10}^{-4}t+1/57.04}$	0.8884	2.46E-4