Table 2.
RT-PCR Primer Pairs
Primer Namesa | Sites (nt) | Sequences (5’~3’)b | Restriction Sites |
---|---|---|---|
5UTR-S | 1~20 | TATCCGCGGTTAAAACAGCCTGTGGGTTG | Sac II |
5UTR-A | 869~888 | GCGGTCGACGGTCTTGAGTGAAATCCTGC | Sac I |
1AB-S | 666~690 | GTCCCCGCGGCTTTGTTGGGTTTATACCACTTAGC | Sac II |
1AB-A | 1925~1949 | GACGCGTCGACAGGTATCTGGTATGCTTCCATAGAG | Sac I |
1C-S | 1603~1627 | GTCCCCGCGGACAACGTCACCCTAATGGTTATCCC | Sac II |
1C-A | 2843~2867 | GACGCGTCGACGTGTGTTAGGATCTGTGCGTCTTGG | Sac I |
1D-S | 2359~2383 | TCCCCGCGGGTTACATCATGTGTTTCGTGTCAGC | Sac II |
1D-A | 3391~3415 | GACGCGTCGACAAGAGGTCTCTGTTGTAACTTTCCC | Sac I |
2AB-S | 3221~3245 | TCCCCGCGGGGCAAAGAACGTGAACTTCCAACCC | Sac II |
2AB-A | 4073~4097 | GACGCGTCGACTTCCATACCCTTGCAAGCATTCGTC | Sac I |
2C-S | 3777~3801 | TCCCCGCGGGCATTCGGCTCCGGCTTTACTAACC | Sac II |
2C-A | 5094~5118 | GACGCGTCGACTGTCTACCGATTTGAGCAGGTCCGC | Sac I |
3ABC-S | 4705~4729 | TCCCCGCGGCCAATGCAGGATCTATTAATGCTCC | Sac II |
3ABC-A | 6015~6039 | GACGCGTCGACTGAGTACTGCTGGTTCTTTGTTCCC | Sac I |
3DUTR-S | 5742~5766 | GTCCCCGCGGGGTGGCACACCCACCAAGAGAATGC | Sac II |
3DUTR-A | 7372~7396 | GACGCGTCGACCGCACCGAATGCGGAGAATTTACCC | Sac I |
“S” designates sense primer, and “A” designates anti-sense primer.
Additional bases were written in italics, and the restriction sites in primer were underlined.