Figure 2.
Tumorigenicity of Hep-2 cell lines in nude mice. The tumorigenicity of Hep-2 cell line co-expression of perforin and granzyme B was tested in BALB/c nu/nu mice. The control animals were treated with pVAX1 transfected Hep-2 cell line or parental Hep-2 cell line. Confluent Hep-2 cells (5 × 106 cells/100 μl) were inoculated subcutaneously into the right flank of mice, and monitored for tumor production (n = 6 per group). Tumor size was measured on days 7, 10, 13, 16, 19, 22, 25, and 28 post inoculation. Data from three animal experiments were pooled. The average tumor volume in cubic centimeters was plotted against time in days. The error bars represent standard deviation. Notice the significant reduction in tumor size in animals inoculated with co-expression of perforin and granzyme B recombinant Hep-2 cell line. A repeated measures analysis of variance demonstrated a significant difference in tumor volume between test and control animals (P < 0.01).