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Western blotting assay in HepG2 cells. ASH1L, C17ORF49 and SETD4 were silencing in HepG2 cells and treated with 5 μmol/L sorafenib or left untreated. 72 h later, cell lysates were subjected to SDS-PAGE and immunoblotted to 0.40 μm of PVDF membrane. And then, GAPDH, ERK, p-ERK, AKT and p-AKT were examined by antibodies.
