Table 1.
Overview over the different binary YA fusion vectors for stable transformation
For each variant, a 35S promoter-based (pBIN) and a UBI10 promoter-based (pBINU) version was constructed. Both versions are available as vectors carrying either kanamycin (K) or Basta (B) resistance.
Subcellular localization | Binary plasmid 35S promoter | UBI10 promoter |
---|---|---|
Cytosol and nucleus | pBIN-YA(K) | pBINU-YA(K) |
pBIN-YA(B) | pBINU-YA(B) | |
Cytosol only | pBIN-CYA(K) | pBINU-CYA(K) |
pBIN-CYA(B) | pBINU-CYA(B) | |
Nucleolus only | pBIN-NYA(K) | pBINU-NYA(K) |
pBIN-NYA(B) | pBINU-NYA(B) | |
Plasma membrane | pBIN-PMYA(K) | pBINU-PMYA(K) |
pBIN-PMYA(B) | pBINU-PMYA(B) | |
Mitochondria | pBIN-MYA(K) | pBINU-MYA(K) |
pBIN-MYA(B) | pBINU-MYA(B) | |
Chloroplast OE surface | pBIN-OEYA(K) | pBINU-OEYA(K) |
pBIN-OEYA(B) | pBINU-OEYA(B) | |
Chloroplast IE surface | pBIN-IEYA(K) | pBINU-IEYA(K) |
pBIN-IEYA(B) | pBINU-IEYA(B) | |
Stroma | pBIN-CHYA(K) | pBINU-CHYA(K) |
pBIN-CHYA(B) | pBINU-CHYA(B) |