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. 2014 Mar 5;4(3):130232. doi: 10.1098/rsob.130232

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© 2014 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 4. — PEP inhibits the catalytic activity of TPI. (a) TPI_Ile170Val and TPI_Ile170Thr have reduced catalytic activity, TPI_Lys13Arg is inactive. Enzyme activity expressed as substrate conversion rate in micromoles per minute and microgram protein. (b) Enzymatic properties of TPI, TPI_Ile170Val, TPI_Ile170Thr and their inhibition by PEP. (c) Substrate titration curves of G3P (black curves, to be read from left to right) on TPI and its mutant enzymes, as well as inhibitor titration curves for PEP (blue curves, to be read from right to left). Substrate/inhibitor saturation was used to calculate V_max, K_m (G3P titrations), and IC₅₀ and K_i values (PEP titrations) (inset table).