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. 2014 Mar 31;34(2):e00101. doi: 10.1042/BSR20130095

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© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) (a) Following induction cell morphology had larger and more apparent nodules as induction time increased. (b) Alizarin Red staining at day 14 post-induction. (c) Control group. (B) RT–PCR assays shown expression of osteoblast-marker genes, including collagen type I, osteopontin, at day 14 post-induction. (d, f) Express osteogenic differentiation marker genes collagen type I, osteopontin. (e, g) Negative control displayed the osteogenic differentiation marker genes were negative. (h) GAPDH as internal control