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. 2014 Mar 31;34(2):e00101. doi: 10.1042/BSR20130095

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© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) Morphology observe (a–c) Control group, P3, P12 and P21 cell morphology (200×). (d–f) Experimental group, P3, P12, P21 cell morphology (200×), Long axons were observed and cells displayed a typical neuron-like morphology. (B) RT–PCR analysis of specific neural markers nestin and MAP-2. (g, i) Induced group shown the neural differentiation marker genes were positive. (h, j) Control group shown the neural differentiation marker genes were negative. (k) GAPDH as internal control.