Table 1. RT-PCR primers sequences of DMSCs.
Gene | Direction | Primer sequences (5′→3′) | Tm (°C) | Product length (bp) | Cycle number |
---|---|---|---|---|---|
CD44 | Forward | CGGAACATAGGGTTTGAGA | 56 | 301 | 30 |
Reverse | GGTTGATGTCTTCTGGGTTA | ||||
CD29 | Forward | GAAACTTGGTGGCATCGT | 58 | 493 | 30 |
Reverse | CTCAGTGAAGCCCAGAGG | ||||
CD73 | Forward | CAATGGCACGATTACCTG | 56 | 428 | 30 |
Reverse | GACCTTCAACTGCTGGATA | ||||
CD34 | Forward | CCTCATCAGCTTTGCGACTT | 60 | 314 | 30 |
Reverse | CCAGGAGCAAGGAGCACA | ||||
CD90 | Forward | CTACCCAACCTTCTACTCAA | 56 | 221 | 30 |
Reverse | TTCACATCCAGGAGGTTC | ||||
CD106 | Forward | GTGAAGGCATTAACCAGG | 55 | 379 | 30 |
Reverse | GCACAATAGAGCACGAGA | ||||
CD166 | Forward | TATCAGGATGCTGGAAAC | 55 | 498 | 30 |
Reverse | TAGCCAATAGACGACACC | ||||
OCT-4 | Forward | CTACCCAACCTTCTACTCAA | 58 | 343 | 30 |
Reverse | TTCACATCCAGGAGGTTC | ||||
GAPDH | Forward | GGCAAGTTCAACGGCACAGTCA | 60 | 364 | 30 |
Reverse | TAAGTCCCTCCACGATGCCAAAG | ||||
Collage type I | Forward | AGAAGCATGTCTGGGTAGGAG | 57 | 358 | 30 |
Reverse | AGGATAGGCAGGCGAGATR | ||||
Osteopontin | Forward | CCAATGAAAGCCCTGAG | 59 | 310 | 30 |
Reverse | TCCTCCTCTGTGGCATC | ||||
PPAR-γ | Forward | ATCCCTGTTCCGTGCTG | 56 | 356 | 30 |
Reverse | GGGATACAGGCTCCACTT | ||||
LPL | Forward | GAACTGGATGGCGGATG | 59 | 256 | 30 |
Reverse | CTGGATTCCGATACTTCGACCT | ||||
Nestin | Forward | ATCCCTGTTCCGTGCTG | 59 | 204 | 30 |
Reverse | GGGATACAGGCTCCACTT | ||||
MAP2 | Forward | GAACTGGATGGCGGATG | 60 | 303 | 30 |
Reverse | CTGGATTCCGATACTTCGACCT |