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. 2014 Mar 31;34(2):e00101. doi: 10.1042/BSR20130095

Table 1. RT-PCR primers sequences of DMSCs.

Gene Direction Primer sequences (5′→3′) Tm (°C) Product length (bp) Cycle number
CD44 Forward CGGAACATAGGGTTTGAGA 56 301 30
Reverse GGTTGATGTCTTCTGGGTTA
CD29 Forward GAAACTTGGTGGCATCGT 58 493 30
Reverse CTCAGTGAAGCCCAGAGG
CD73 Forward CAATGGCACGATTACCTG 56 428 30
Reverse GACCTTCAACTGCTGGATA
CD34 Forward CCTCATCAGCTTTGCGACTT 60 314 30
Reverse CCAGGAGCAAGGAGCACA
CD90 Forward CTACCCAACCTTCTACTCAA 56 221 30
Reverse TTCACATCCAGGAGGTTC
CD106 Forward GTGAAGGCATTAACCAGG 55 379 30
Reverse GCACAATAGAGCACGAGA
CD166 Forward TATCAGGATGCTGGAAAC 55 498 30
Reverse TAGCCAATAGACGACACC
OCT-4 Forward CTACCCAACCTTCTACTCAA 58 343 30
Reverse TTCACATCCAGGAGGTTC
GAPDH Forward GGCAAGTTCAACGGCACAGTCA 60 364 30
Reverse TAAGTCCCTCCACGATGCCAAAG
Collage type I Forward AGAAGCATGTCTGGGTAGGAG 57 358 30
Reverse AGGATAGGCAGGCGAGATR
Osteopontin Forward CCAATGAAAGCCCTGAG 59 310 30
Reverse TCCTCCTCTGTGGCATC
PPAR-γ Forward ATCCCTGTTCCGTGCTG 56 356 30
Reverse GGGATACAGGCTCCACTT
LPL Forward GAACTGGATGGCGGATG 59 256 30
Reverse CTGGATTCCGATACTTCGACCT
Nestin Forward ATCCCTGTTCCGTGCTG 59 204 30
Reverse GGGATACAGGCTCCACTT
MAP2 Forward GAACTGGATGGCGGATG 60 303 30
Reverse CTGGATTCCGATACTTCGACCT