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. Author manuscript; available in PMC: 2014 Apr 9.
Published in final edited form as: Stem Cells Dev. 2013 Mar 5;22(12):1851–1860. doi: 10.1089/scd.2012.0658

Figure 1. Analysis of the side scatter parameter in pluripotent stem cells (PSCs) and human blood cells.

Figure 1

Scatter plot of SSC versus FSC values in HD83 human ES cells. (A) Gating strategy. To quantify the PSCs, we eliminated aggregated cells (gate 1) and dead cells using Sytox blue stain (gate 2). Debris cells were evaluated based on FSC and SSC. PSCs are in pink. (C) Scatter plot of SSC versus FSC values in human blood cells. Based on the SSC and FSC distribution, granulocytes (orange) can be differentiated from monocytes (blue) and lymphocytes (green). (D) Comparison of the SSC distributions of PSCs, human blood leucocytes, granulocytes and mononuclear cells. Flow cytometry data were imported in the R statistical software using the flowCore package. The SSC distribution of each cell subpopulation was extracted and represented as a dot plot.

SSC: side scatter; FSC: forward scatter; PMN: granulocytes; Mono: mononuclear cells; Lympho: lymphocytes; Leu: leukocytes; MNC: mononuclear cells.