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. Author manuscript; available in PMC: 2014 Apr 9.
Published in final edited form as: Stem Cells Dev. 2013 Mar 5;22(12):1851–1860. doi: 10.1089/scd.2012.0658

Figure 5. Cell cycle distribution and apoptosis rate in HSSC and LSSC cells.

Figure 5

Apoptosis rates and cell cycle distribution were analyzed in M4C7 cells (hiPSCs). (A) Representative dot plot of the Annexin-V- and Sytox Blue-stained PSCs. Fibroblast feeder cells were excluded by labeling with anti-CD44 antibodies. (B) Quantification of live, dead, early and late apoptotic cells in the HSSC and LSSC subpopulations indicates that HSSC cells have higher apoptosis rates than LSSC cells. (C and D) M4C7 cells were stained with propidium iodide and the anti-CD44 antibody to exclude fibroblast feeder cells and the cell cycle distribution quantified in HSCC and LSSC cells. Data are the mean ± SEM of three independent experiments.