Table 2.
Computational analysis of densitometric data (*density data from a control sample loaded in the first lane of each gel/blot for a given study).
| Biological replicates | Sample | Density (volume) | |||
|---|---|---|---|---|---|
| Target protein (TP) | Loading control (LC) | Normalized density to LC (NDL) (TP* LC/LC*) | Fold difference (NDL/NDL*) | ||
| M1 | T = 0 | 8 | 4* | (8 × 4/4) = 8.0* | (8/8) = 1.0 |
| M1 | T = 1 | 45 | 5 | (45 × 4/5) = 36.0 | (36/8) = 4.5 |
| M1 | T = 2 | 90 | 6 | (90 × 4/6) = 60.0 | (60/8) = 7.5 |
| M2 | T = 0 | 6 | 5 | (6 × 4/5) = 4.8 | (4.8/8) = 0.6 |
| M2 | T = 1 | 40 | 5 | (40 × 4/5) = 32.0 | (32/8) = 4.0 |
| M2 | T = 2 | 88 | 7 | (88 × 4/7) = 50.3 | (50.3/8) = 6.3 |
| M3 | T = 0 | 10 | 4 | (10 × 4/4) = 10.0 | (10/8) = 1.3 |
| M3 | T = 1 | 48 | 6 | (48 × 4/6) = 32.0 | (32/8) = 4.0 |
| M3 | T = 2 | 92 | 6 | (92 × 4/6) = 61.3 | (61.3/8) = 7.7 |