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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Am J Transplant. 2014 Jan 9;14(2):343–355. doi: 10.1111/ajt.12560

Figure 1. Reduced intensity conditioning regimen for induction of mixed chimerism and VCA tolerance.

Figure 1

Recipients underwent 100 cGy TBI and T cell depletion with CD3-Immunotoxin (CD3-IT) prior to hematopoietic cell transplantation (HCT) with 15 × 109 cells/kg in two to three doses over days 0–2 as required to achieve target cell number. Cyclosporine A (CyA) was administered commencing on days −1 to 30 (target trough 400–800 ng/mL) followed by a taper to discontinuation on day 45. VCA transplantation was performed into stable chimeras between 85 and 150 days post-HCT, or simultaneous to induction of mixed chimerism, within 56 h of the first infusion of donor hematopoietic cells. *CD3-IT was administered either as the conjugate pCD3-CRM9 as a single 50 μg/kg dose on day −2 (animals 17468 and 17469) or as eight 50 μg/kg doses of recombinant pCD3-DT390, twice a day from day −4 to −1 (animals 20311, 20313, 20680 and 20681). TBI, total body irradiation; VCA, vascularized composite allograft.