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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Am J Transplant. 2014 Jan 9;14(2):343–355. doi: 10.1111/ajt.12560

Figure 7. Lack of evidence for IL-2 reversible anergy as the dominant mechanism of tolerance following HCT and VCA.

Figure 7

(A) Recipients of HCT and VCA together developed donor-specific unresponsiveness in vitro as early as 2–4 weeks posttransplant. Representative data from 20681 at week 4 are shown. (B) Addition of exogenous porcine IL-2 to MLR failed to restore anti-donor responses. (C, D) The conditioned control animal 20989 maintained anti-donor and anti-third party both with and without IL-2. Addition of IL-2 promotes a general increase in proliferation in MLR; therefore, proliferation in IL-2 assays is expressed as the delta-counts per minute (CPM) in comparison to the anti-self response. HCT, hematopoietic cell transplantation; MLR, mixed lymphocyte reaction assay; VCA, vascularized composite allograft.